Dushku was born in Watertown, Massachusetts, the daughter of Philip Richard George Dushku, an administrator and teacher in the Boston Public Schools, and Judith Ann "Judy" Dushku (née Rasmussen), a political science professor.
Dushku came to the attention of casting agents when she was 10.
She appeared at Spike TV's 2008 Video Game Awards in December 2008.
She quit in February 2006 along with other members of the cast amidst rumors of abuse from the producer, which were latery-drama for Fox.but her agent asked her to submit a videotape audition for a show starring another of his clients, Sarah Michelle Gellar.After reading the script, Dushku rushed to a local Claire's to purchase dark makeup and other appropriate accessories for the part, Faith on Buffy the Vampire Slayer.Repentant and rededicated, Faith returned as a heroine in other episodes of Angel and in the last five episodes of Buffy.Dushku was inundated with piles of fan mail from legions of prisoners." – and there's some guy sat on the sofa with a bottle of beer and a moustache, and a big gut. Dushku turned down a role in a spin-off of Buffy The Vampire Slayer which would have been about Faith.She has had many roles as a "bad girl" in movies and relishes the opportunities.She said: I've been getting fan mail from maximum security penitentiaries and death row.What are the authorities thinking of in playing a show with young teenage girls to Death Row inmates?The movie was released in Los Angeles on November 30, 2007, and on DVD on January 22, 2008.She starred in Open Graves, a 2008 horror-thriller about a satanic game co-starring Mike Vogel.