Mass-spectrometry data were produced using a SWATH acquisition mode on a Sciex Triple-TOF 6600.
A spectral library built in-house was used to analyse these data and more than 1950 proteins were quantified at each embryonic timepoint.
Although the first generation of 1,237 offspring was all red-eyed but for three, white-eyed flies appeared in larger numbers in the second generation. These results were suggestive for hypotheses of which Morgan himself was skeptical.
In this dataset, we monitor changes in protein expression across a timecourse of more than 20 h of embryonic development.All first-generation offspring of a mutant white-eyed male and a normal red-eyed female would have red eyes because every chromosome pair would contain at least one copy of the X chromosome with the dominant trait.But half the females from this union would now possess a copy of the white-eyed male's recessive X chromosome.The shape of one of four chromosome pairs was thought to be distinctive for sex determination.Males invariably possess the XY chromosome pair (Morgan used a more cumbersome notation) while flies with the XX chromosome are female.He thereby inaugurated classical experimental genetics. in his laboratory at Columbia University, in 1910 Morgan noticed one fruit fly with a distinctive characteristic: white eyes instead of red.He isolated this specimen and mated it to an ordinary red-eyed fly.Embryogenesis is one of the most important processes in the life of an animal.During this dynamic process, progressive cell division and cellular differentiation are accompanied by significant changes in protein expression at the level of the proteome.Such a sexual interaction was highly consistent from one pair to another (Fig. (A) Copulation success of wild-type (CS) in the circular arenas. Notes: The red piece in the pie chart indicates copulation success.Each pair of flies (a naive male and a virgin female) were loaded into a circular arena (1.27 cm diameter, 0.3 cm depth), and their copulation activities within 60 minutes were examined. (B) A defect of copulation success in w In CS flies, the percentage of copulation success was 82.5% (33/40) (Fig. Median copulation duration was 25.4 min (interquartile range (IQR) 22.3–28.6 min) with a median latency of 11.1 min (IQR 6.6–19.3 min) (Fig. A pair of flies comprise a naive male and a virgin female. Numbers of pairs tested and pairs with successful copulation are shown. flies require longer than an hour to start copulation.